Reporter

Part:BBa_K2121013:Design

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


AStr_Citrine_T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1914
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1881
    Illegal BsaI.rc site found at 55


Design Notes

Strong RBS that elevates citrine expression level in rhizobia.

Construct design

Design_1.png

Design_2.png


Source

Anderson promoter and T1 terminator from iGEM registry. Citrine sequence from Stephen Dellaporta Lab, Yale University.

References