Coding

Part:BBa_K2121002:Design

Designed by: Jinny van Doorn   Group: iGEM16_Yale   (2016-10-20)


Lambda_Beta_B5ZVM8_RHILW (red_rhizo-MAGE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 871
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 769


Design Notes

Elimination of any illegal restriction sites, and codon optimization for expression in S. meliloti 1021 and R. tropici CIAT 899.


Source

Gene Rleg2_2465 phage for Rhizobium leguminosarum bv. trifolii (strain WSM2304)

References