Device
Part:BBa_K2120301:Design
Designed by: HongYu Yang Group: iGEM16_BIT-China (2016-10-13)
pR+B0032+RFP+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 631
Illegal AgeI site found at 743 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The PR promoter has two DNA binding sites for lambada CI repressor BBa_C0051.and RFP is the reporter gene to test the inhibit efficiency of PR promoter.In our project,the reporter gene is regarded as the killer gene.We use it in our validation circuit.