Signalling
Part:BBa_K212006:Design
Designed by: Elias Scheer Group: iGEM09_Brown (2009-10-20)
mRBP (modified ribose binding protein - now binds histamine)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed using Rosetta 3 macromolecular modeling program.
Source
synthesized DNA incorporating 16 mutations from native E. coli RBP gene.
References
1. Looger et al. Computational design of a receptor and sensor proteins with novel functions. Nature, 2003.
2. Mowbray et al. 1.7 Å X-ray structure of the periplasmic ribose receptor from Escherichia coli. J. Mol. Bio., 1992.