Signalling

Part:BBa_K212006:Design

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)

mRBP (modified ribose binding protein - now binds histamine)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed using Rosetta 3 macromolecular modeling program.

Source

synthesized DNA incorporating 16 mutations from native E. coli RBP gene.

References

1. Looger et al. Computational design of a receptor and sensor proteins with novel functions. Nature, 2003.

2. Mowbray et al. 1.7 Å X-ray structure of the periplasmic ribose receptor from Escherichia coli. J. Mol. Bio., 1992.