Part:BBa_K212001:Design
Tar-Envz (Taz)
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 420
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 420
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 420
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 111
Design Notes
This is a chimeric two-component transduction system, so we had to be careful to avoid cross-talk by making sure that EnvZ phosphorylates OmpR only.
Source
Tar-EnvZ (Taz) is a chimera protein, manufactured by Inouye, et al. We obtained the gene from his lab and Biobricked it. Taz comprises of the aspartate chemoreceptor region of Tar, the transmembrane region of Tar, and the intracellular kinase region of EnvZ. The genes were fused by digesting both with NdeI and ligating the overlapping ends together. The cut site is between amino acids H256 and M257.
References
1.
2. Zhu, Yan, and Masayori Inouye. "Analysis of the Role of the EnvZ Linker Region in Signal Transduction Using a Chimeric Tar/EnvZ Receptor Protein, Tez1." The Journal of Biological Chemistry 278.25 (2003): 22812-2819. Print.