Composite

Part:BBa_K2120002:Design

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-12)


araC+pBAD+B0032+mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The 2016 BIT-China iGEM Team equipped the bacteria with a plasmid-sensing logically adjustable cell killer(P-SLACKiller). In our project, we use MazF, a kind of toxin protein can cut specific ACA sequence of free mRNA, to kill the slacker of culture. This part(araC+pBAD+B0032+mazF) is used to verify the function of the toxin protein, MazF



Source

E.coli MG1655

References