Composite
Part:BBa_K2120002:Design
Designed by: HongYu Yang Group: iGEM16_BIT-China (2016-10-12)
araC+pBAD+B0032+mazF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The 2016 BIT-China iGEM Team equipped the bacteria with a plasmid-sensing logically adjustable cell killer(P-SLACKiller). In our project, we use MazF, a kind of toxin protein can cut specific ACA sequence of free mRNA, to kill the slacker of culture. This part(araC+pBAD+B0032+mazF) is used to verify the function of the toxin protein, MazF
Source
E.coli MG1655