Regulatory

Part:BBa_K2119131:Design

Designed by: Macarena Natalia Ramos Briones   Group: iGEM16_Saint_Rose_School_B   (2016-10-14)


Cellobiose response regulator (ClbR) is a Zn(II)2Cys6 transcriptional activator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 669
    Illegal BamHI site found at 1701
    Illegal BamHI site found at 1831
    Illegal XhoI site found at 877
    Illegal XhoI site found at 1616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 2272
    Illegal AgeI site found at 946
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2362
    Illegal SapI.rc site found at 428


Design Notes

yes, the only problem was finding the coding sequence at the NCBI database


Source

the source of this part is the genomic database of the NCBI, and this sequence comes from Aspergillus acelatus.

References