Device

Part:BBa_K2118004:Design

Designed by: Laura Ros-Freixedes   Group: iGEM16_UPF-CRG_Barcelona   (2016-10-14)


J23119 + FMS1 gene + PatA gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1984
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 217
    Illegal AgeI site found at 900
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1350
    Illegal AgeI site found at 2300
    Illegal AgeI site found at 2441
    Illegal AgeI site found at 2456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we have two rbs so as to improve the transcription rate of the second gene


Source

IDT

References