Coding
Part:BBa_K2114015:Design
Designed by: Wladislaw Stroukov Group: iGEM16_Freiburg (2016-10-09)
GST_HA_G4S_CotB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1750
Illegal BamHI site found at 688 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Illegal SapI.rc site found at 1204
Design Notes
Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR.
Source
The cgeA gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389–2401 (2010).