Coding
Part:BBa_K2114014:Design
Designed by: Wladislaw Stroukov Group: iGEM16_Freiburg (2016-10-09)
GST_HA_aHelix_CotG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Design Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR.
Source
The cotB gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies described by Kubala, M. H., Kovtun, O., Alexandrov, K. & Collins, B. M. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci. 19, 2389–2401 (2010).