Coding

Part:BBa_K2114007:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-08)


aGFPnano_HA_G4S_CotG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.

Source

The cotG gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies.

References