Primer
Part:BBa_K2110829:Design
Designed by: Haipeng Yu Group: iGEM16_Tianjin (2016-10-05)
PETase site mutation S207A - F
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.
Source
Artificially Designed