Composite

Part:BBa_K2109105:Design

Designed by: Andy Hudson   Group: iGEM16_Lethbridge   (2016-10-14)

To simplify optimization of our bacterial-2-hybrid system we chose to host our RNA polymerase-target expressing construct on the same plasmid as our reporter construct. However, to avoid undesirable activation of our reporter, we placed the RNA polymerase construct downstream.


B2HR-RNAPa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 130
    Illegal NheI site found at 153
    Illegal NheI site found at 1822
    Illegal NheI site found at 3045
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3895
    Illegal BamHI site found at 84
    Illegal BamHI site found at 2984
    Illegal XhoI site found at 118
    Illegal XhoI site found at 3931
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3603
    Illegal AgeI site found at 2819
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2801


Design Notes

n/a


Source

n/a

References