Generator

Part:BBa_K2107001:Design

Designed by: Ellen Wilson   Group: iGEM16_Lubbock_TTU   (2016-10-19)


Stationary Phase Promoter + Kil Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 99
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part should be cloned into a low to medium copy plasmid for the best expression.



Source

GAA TTC GCG GCC GCT TCT AGA GCA ATC TTG CCC GCG CTT CTG CTC TCC CGG CGT AAC CCG GAT TTG CCG CTT ATA CTT GTG GCA AAT GGA CAC GTT CAG GGA GTA GAG AAA GAG GGA CAA ACT AGT ATG ATG CGT AAC GAA ATC GCG ATC AAC CAC CAG ATG CTG CGT GCG GCG CAG AAC AAA GCG GTT ATC GCG CGT TTC ATC GGT GAC TCT AAA ATG TGG CTG GAA GCG AAC AAA GCG ATG AAA TCT GCG ATC AAC CTG CCG TGG TAC CGT CGT AAA TAA TGA TAC TAG TAG CGG CCG CTG CAG

References