Project

Part:BBa_K2103001:Design

Designed by: Maggie Chen   Group: iGEM16_CCA_SanDiego   (2016-10-12)


NADPH P450 Reductase (Homo Sapiens)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 659
    Illegal NgoMIV site found at 778
    Illegal NgoMIV site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 1404
    Illegal SapI.rc site found at 785


Design Notes

Oligo bridges between the restriction sites were noncoding and annealed solely for the purpose of standardizing the plasmid backbone.


Source

Homo Sapiens

References