Part:BBa_K2100072:Design

pEXPR hEF1a-SV40: eYFP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 29
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 1262
  Illegal PstI site found at 360
  Illegal PstI site found at 865
  Illegal PstI site found at 1762
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 29
  Illegal EcoRI site found at 1233
  Illegal PstI site found at 360
  Illegal PstI site found at 865
  Illegal PstI site found at 1762
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 29
  Illegal EcoRI site found at 1233
  Illegal BglII site found at 614
  Illegal BamHI site found at 1220
  Illegal XhoI site found at 1013
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 29
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 1262
  Illegal PstI site found at 360
  Illegal PstI site found at 865
  Illegal PstI site found at 1762
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 29
  Illegal EcoRI site found at 1233
  Illegal XbaI site found at 1262
  Illegal PstI site found at 360
  Illegal PstI site found at 865
  Illegal PstI site found at 1762
  Illegal NgoMIV site found at 748
  Illegal AgeI site found at 126
  Illegal AgeI site found at 1545
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

We synthesized this part from two entry vectors.