Part:BBa_K2100071:Design

pEXPR hEF1a: attB-flipped eYFP-attP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal EcoRI site found at 3389
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3098
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal EcoRI site found at 3389
  Illegal NheI site found at 2071
  Illegal NheI site found at 2337
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3098
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal EcoRI site found at 3389
  Illegal BglII site found at 591
  Illegal BamHI site found at 1197
  Illegal BamHI site found at 2501
  Illegal XhoI site found at 990
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal EcoRI site found at 3389
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3098
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1210
  Illegal EcoRI site found at 3389
  Illegal PstI site found at 337
  Illegal PstI site found at 842
  Illegal PstI site found at 3098
  Illegal NgoMIV site found at 725
  Illegal AgeI site found at 103
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1948
  

Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

We synthesized this part from two entry vectors. hEF1a comes from the mammalian genome.