DNA

Part:BBa_K2100067:Design

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR hEF1a: VgEcR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 1517
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 3498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal NheI site found at 3317
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 3498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 591
    Illegal BglII site found at 2811
    Illegal BamHI site found at 1197
    Illegal BamHI site found at 1712
    Illegal BamHI site found at 4188
    Illegal BamHI site found at 4341
    Illegal BamHI site found at 4455
    Illegal XhoI site found at 990
    Illegal XhoI site found at 2804
    Illegal XhoI site found at 2885
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 1517
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 3498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal XbaI site found at 1517
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal PstI site found at 3498
    Illegal NgoMIV site found at 725
    Illegal NgoMIV site found at 2933
    Illegal NgoMIV site found at 3280
    Illegal NgoMIV site found at 4753
    Illegal AgeI site found at 103
    Illegal AgeI site found at 4041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4226
    Illegal SapI.rc site found at 1615
    Illegal SapI.rc site found at 1747


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

We synthesized this part from entry vectors.

References