Generator

Part:BBa_K210005:Design

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-13)

LacI generator yeast-optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This CDS has SV40 nuclear localization sequence. But, due to frameshift, this does not successfully translate.


Source

BBa_K165037 BBa_C0012 BBa_K165018

References

http://en.wikipedia.org/wiki/Kozak_consensus_sequence