Part:BBa_K2100052:Design

pEXPR pERE6:TP901

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 773
  Illegal EcoRI site found at 1876
  Illegal XbaI site found at 42
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 773
  Illegal EcoRI site found at 1876
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 773
  Illegal EcoRI site found at 1876
  Illegal BglII site found at 1656
  Illegal BamHI site found at 360
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 773
  Illegal EcoRI site found at 1876
  Illegal XbaI site found at 42
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 773
  Illegal EcoRI site found at 1876
  Illegal XbaI site found at 42
  Illegal NgoMIV site found at 524
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

This is from a synthetic promoter and a gene from the mammalian genome.