![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100045:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pTALER14:mKate
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal BamHI site found at 303
Illegal XhoI site found at 246 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal XbaI site found at 157 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 318
Illegal BsaI.rc site found at 999
Illegal SapI.rc site found at 381
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is a mammalian promoter with a gene from the anemone genome.