Part:BBa_K2100044:Design

pEXPR pERE6:BM3R1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 749
  Illegal XbaI site found at 42
  Illegal PstI site found at 566
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 749
  Illegal NheI site found at 983
  Illegal PstI site found at 566
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 749
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 749
  Illegal XbaI site found at 42
  Illegal PstI site found at 566
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 749
  Illegal XbaI site found at 42
  Illegal PstI site found at 566
  Illegal NgoMIV site found at 488
  Illegal NgoMIV site found at 723
  Illegal AgeI site found at 501
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

This is a synthetic promoter with a gene from the mammalian genome.