DNA

Part:BBa_K2100042:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pERE3:BM3R1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 596
    Illegal PstI site found at 413
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 596
    Illegal NheI site found at 830
    Illegal PstI site found at 413
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 596
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 596
    Illegal PstI site found at 413
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 596
    Illegal PstI site found at 413
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 570
    Illegal AgeI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter with a gene from a mammalian genome.

References