Part:BBa_K2100035:Design

pEXPR pERE6:TALER14

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 539
  Illegal XbaI site found at 42
  Illegal XbaI site found at 516
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 539
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 539
  Illegal BamHI site found at 662
  Illegal XhoI site found at 605
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 539
  Illegal XbaI site found at 42
  Illegal XbaI site found at 516
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 18
  Illegal EcoRI site found at 333
  Illegal EcoRI site found at 343
  Illegal EcoRI site found at 365
  Illegal EcoRI site found at 539
  Illegal XbaI site found at 42
  Illegal XbaI site found at 516
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 677
  

Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

This is a synthetic promoter with a gene from the mammalian genome.