DNA

Part:BBa_K210002:Design

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-09)

ARS1 and CEN4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 233
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 447
    Illegal PstI site found at 233
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 262
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 233
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 233
    Illegal NgoMIV site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has Pst1 restriction enzyme sites.


Source

pYAC4

References

pYAC4 Neo, a yeast artificial chromosome vector which codes for G418 resistance in mammalian cells

Howard Cooke and Sally Cross