DNA

Part:BBa_K2100008:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)


pENTR pPRE3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 211
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 225


Design Notes

This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter from IDT.

References