Composite

Part:BBa_K2097000:Design

Designed by: Sofia Chinea   Group: iGEM16_Austin_UTexas   (2016-09-13)


CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence directly from the papers - "Involvement of cpxA, a Sensor of a Two-Component Regulatory System, in the pH-Dependent Regulation of Expression of Shigella sonnei virF Gene" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177285/pdf/1775062.pdf) and "Identification of cpxR as a Positive Regulator Essential for Expression of the Shigella sonnei virF Gene" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107317/pdf/jb003522.pdf) - was not used. We used the loci detailed in the papers and found the CpxR binding sequence in the GenBank to construct the sequenced used in the experiments.


Source

The two parts together (CpxR binding site - YGCP) were ordered from IDT.

The original sources for the CpxR binding site came from:

"Involvement of cpxA, a Sensor of a Two-Component Regulatory System, in the pH-Dependent Regulation of Expression of Shigella sonnei virF Gene" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177285/pdf/1775062.pdf

"Identification of cpxR as a Positive Regulator Essential for Expression of the Shigella sonnei virF Gene" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107317/pdf/jb003522.pdf

GenBank used: http://www.ncbi.nlm.nih.gov/nuccore/CP000039.1?&feature=CDS

The YGCP (Bba_K1033916) comes from the iGEM Registry.

References