Part:BBa_K209473:Design

pcDNA3.1(+) with AarI A-D sites in MCS

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 1065
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2387
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal NheI site found at 895
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2387
  Illegal NotI site found at 978
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal BglII site found at 12
  Illegal BamHI site found at 929
  Illegal XhoI site found at 1059
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 1065
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2387
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 1065
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2387
  Illegal NgoMIV site found at 1497
  Illegal NgoMIV site found at 2838
  Illegal NgoMIV site found at 3121
  Illegal AgeI site found at 1013
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 882
  Illegal BsaI.rc site found at 4646
  Illegal SapI site found at 3566
  Illegal SapI.rc site found at 2687
  Illegal SapI.rc site found at 2897
  

Source

Parent plasmid from Invitrogen.