Coding

Part:BBa_K209467:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI B-C part, EDG1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1045
    Illegal PstI site found at 663
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1045
    Illegal PstI site found at 663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1045
    Illegal PstI site found at 663
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1045
    Illegal PstI site found at 663
    Illegal NgoMIV site found at 1036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 45
    Illegal BsaI.rc site found at 1108
    Illegal SapI site found at 813


Design Notes

Cloned into pCR2.1.

Source

Plasmid obtained from [http://www.cdna.org/G-Protein-Coupled-Receptors-c8.html Missouri S&T cDNA Resource Center].

References