Coding

Part:BBa_K209460:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI B-C part, ADRA1A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1038
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1038
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1038
    Illegal NgoMIV site found at 51
    Illegal NgoMIV site found at 501
    Illegal AgeI site found at 57
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 427
    Illegal BsaI.rc site found at 517
    Illegal BsaI.rc site found at 1147
    Illegal SapI site found at 555


Design Notes

Cloned into pCR2.1.

Source

Plasmid obtained from [http://www.cdna.org/G-Protein-Coupled-Receptors-c8.html Missouri S&T cDNA Resource Center].

References