Composite

Part:BBa_K209450:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-Rs1.3-ActA(255-392)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 1952
    Illegal BamHI site found at 2166
    Illegal XhoI site found at 1479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84


Design Notes

Cloned into pEF5/FRT/V5-DEST.


Source

References