Composite

Part:BBa_K209443:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)

pEF1-SSFYFP-Rs1.3-LPD(775-1250)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 2646
    Illegal PstI site found at 3021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
    Illegal PstI site found at 2646
    Illegal PstI site found at 3021
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 1952
    Illegal BamHI site found at 2166
    Illegal XhoI site found at 1479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 2646
    Illegal PstI site found at 3021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 2646
    Illegal PstI site found at 3021
    Illegal AgeI site found at 2527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI site found at 2991
    Illegal BsaI.rc site found at 2938
    Illegal BsaI.rc site found at 3005
    Illegal BsaI.rc site found at 3678


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References