Coding

Part:BBa_K2094001:Design

Designed by: Kyujin Jeong   Group: iGEM16_Korea_U_Seoul   (2016-10-07)


Diaphorase (cytochrome b5 reductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
    Illegal AgeI site found at 617
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None. It was simply synthesized after codon optimization and cloned into pSB1C3.

Source

The sequence comes from sus scrofa(pig). It was codon optimized to E.coli K-12 and was synthesized.

References

https://www.ncbi.nlm.nih.gov/protein/39931643?report=genbank&log$=protalign&blast_rank=1&RID=ZV2JDRGJ014