Coding

Part:BBa_K2091004:Design

Designed by: I. Bariah, E. Zajfman, I. Segal   Group: iGEM16_BGU_ISRAEL   (2016-10-03)


LC-Cutinase Codon Optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was altered using the Genome Compiler software so the codons are optimized for E. coli.
We have also introduced 4 point mutations so the sequence doesn't include cut sites for EcoRI and PstI. The sequence was synthesized by Syntezza Bioscience.

Source

This part is based on the LC-Cutinase protein2. The wild-type gene was acquired from the 2012 UC-Davis iGEM team. (BBa_K936000)

References

1. Schwarz, C. K., Landsberg, C. D., Lenders, M. H., Smits, S. H., & Schmitt, L. (2012). Using an E. coli Type 1 secretion system to secrete the mammalian, intracellular protein IFABP in its active form. Journal of biotechnology, 159(3), 155-161.‏
2. Sulaiman, S., Yamato, S., Kanaya, E., Kim, J. J., Koga, Y., Takano, K., & Kanaya, S. (2012). Isolation of a novel cutinase homolog with polyethylene terephthalate-degrading activity from leaf-branch compost by using a metagenomic approach. Applied and environmental microbiology, 78(5), 1556-1562.‏