Coding

Part:BBa_K2091000:Design

Designed by: Ben Vaknin   Group: iGEM16_BGU_ISRAEL   (2016-09-18)


Protocatechuate 3,4-dioxygenase Alpha Subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
    Illegal NgoMIV site found at 79
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 583


Design Notes

Designed using Benchling to meet registry standards.
The sequence was amplified from the P. putida KT2440 strain and cloned into the pSB1C3 vector using EcoRI and PstI.

Source

Genomic DNA of Pseudomonas putida strain KT2440.

References

1. Frazee, R. W., Livingston, D. M., LaPorte, D. C., & Lipscomb, J. D. (1993). Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3, 4-dioxygenase genes. Journal of bacteriology, 175(19), 6194-6202.‏
2. Bull, C., & Ballou, D. P. (1981). Purification and properties of protocatechuate 3, 4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry. Journal of Biological Chemistry, 256(24), 12673-12680.‏