Generator

Part:BBa_K2082221:Design

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-08)


Fusion protein HA4:cMyc:RpoZ generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Badran et al. (2016) and Wojcik et al. (2010) were the main inspiration sources for creating this part.


Source

The fusion protein was created with gene synthesis of IDT.

References

  • Badran, A. H., Guzov, V. M. & Huai, Q. et al. (2016) Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Nature 533 (7601), 58–63.
  • Wojcik, J., Hantschel, O. & Grebien, F. et al. (2010) A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain. Nature structural & molecular biology 17 (4), 519–527.