Coding
Part:BBa_K2082203:Design
Designed by: Pascal Schmidt Group: iGEM16_Bielefeld-CeBiTec (2016-10-07)
Mutated fusion protein HA4(R38A):cMyc:RpoZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Badran et al. (2016) and Wojcik et al. (2010) were the main inspiration sources for creating this part.
Source
The fusion protein was created with gene synthesis of IDT.
References
- Badran, A. H., Guzov, V. M. & Huai, Q. et al. (2016) Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Nature 533 (7601), 58–63.
- Wojcik, J., Hantschel, O. & Grebien, F. et al. (2010) A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain. Nature structural & molecular biology 17 (4), 519–527.