Device

Part:BBa_K2082119

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-16)


AraC-Pbad(mod)-RBS-WTPolI-Ter

Wild type polymerase I BBa_K2082107 under control of PBAD BBa_K2082112


This part should be used as control to BBa_B2082118 to show if it is possible to increase the basal mutagensis of the error prone polymerase I.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1947
    Illegal XhoI site found at 2445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1347
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2910


[edit]
Categories
Parameters
None