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Part:BBa_K2082006:Design

Designed by: Marius Schöller   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Nanobody library: Fusion proteins of Nanobody and omega subunit of RNA polymerase (rpoZ)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli


Source

Derived from single monomeric variable antibody domain of camelids.

References