Device

Part:BBa_K2082003

Designed by: Judith Kampa   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Split CFP Bimolecular fluorescence complementation (BiFC) with GFP

Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein (CFP) CDS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 86
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a RBS (BBa_B0034)
  • a GFP CDS
  • a C-terminal split-CFP CDS

This device can be used in combination with BBa_K2082002 to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.
E. coli with BBa_K2082003. Green fluorescence, caused by GFP.


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