Intermediate

Part:BBa_K208019:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

OmpA Signal Peptide/GFP (Hawaii08 Silver)/Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 712


Design Notes

OmpA secretion tag, Hawaii's 2008 (Silver version) GFP coding, and two terminators in pSB1AK3 vector. Meant for GFP secretion.

Source

Composite

References