Regulatory

Part:BBa_K208013:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-12)

Tet Repressible Promoter (BBa_R0040) and RBS (BBa_B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a Tet promoter with an attached ribosome binding site. The RBS was attached to the promoter and made into a BioBrick to simplify the composite part construction process as cutting and ligating a small sequence, like a ribosome binding site, can be difficult.

Source

This part was designed by the USU iGEM 2009 team and synthesized by DNA 2.0. The part was cut and ligated into a BioBrick plasmid, pSB1AK3 and sequenced.

References

Please consult the original pages for these parts for more details on their functionality.