Coding

Part:BBa_K2074998:Design

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-14)


cry11Aa suit for Chlamydomonas reinhardti


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal PstI site found at 1943
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 1687
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 250
    Illegal BsaI.rc site found at 1465
    Illegal SapI.rc site found at 1446


Design Notes

To identify there is no restriction enzyme cutting site which we choosed.


Source

DNA from a synthesis company.

References