Coding

Part:BBa_K2074027

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)


Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt1(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

Cry4Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt.) which have toxicity to the larvae of mosquitoes.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquitoes.

Fig.1 Cry4Aa and Cyt1 Toxins Group
K2074027.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
    Illegal BglII site found at 3797
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 3631
    Illegal NgoMIV site found at 3931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 2071
    Illegal BsaI.rc site found at 2611
    Illegal BsaI.rc site found at 2920
    Illegal BsaI.rc site found at 3487
    Illegal SapI.rc site found at 1188
    Illegal SapI.rc site found at 1230


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Categories
Parameters
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