Composite

Part:BBa_K2073012:Experience

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)

Applications of BBa_K2073012

Firstly, we aimed to know the effect of fluoride on E.coli. We cultured E.coli in LB with different sodium fluoride concentration and traced the growth curve of E.coli in fifteen hours.

Fluoride_tolerance.png

The result shows that fluoride significantly inhibits the growth of E.coli. The higher fluoride concentration , the less E.coli grow.
In the LB with 0.1M sodium fluoride, the E.coli stops growing.


Secondly, we tested the protein function of Crcb-La1. After transfecting Crcb-La1 gene into E.coli, we cultured them in the LB with 0.1M sodium fluoride.
The positive control is E.coli cultured in LB without sodium fluoride.
The negative control is E.coli without Crcb-La1 gene cultured in LB with 0.1M sodium fluoride

20161030203320%21Crcb-la1.png

Based on the result, in the LB with 0.1M sodium fluoride, the E.coli with Crcb-La1 gene can still grow. (Crcb-La1-test 1,2) However, as for the E.coli without Crcb-La1 gene, the growth is completely inhibited.(negative control)
We can know the Crcb-La1 successfully exfluxes the fluoride and promotes the growth of E.coli.

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