Composite

Part:BBa_K2073012:Design

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


CrcB-La1 with IPTG promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 320
    Illegal AgeI site found at 416
    Illegal AgeI site found at 587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312


Design Notes

After the degradation of PFOA, fluoride would be released simultaneously. To prevent our E. coli get poisoned by excess fluoride, we transfected Crcb(La1) gene into it.
This biobrick can help our E. coli expulse fluoride so that them can survive and grow better.


Source

E.coli

References