Composite

Part:BBa_K2073009:Design

Designed by: WEI-CHIA HSU   Group: iGEM16_NTHU_Taiwan   (2016-10-14)


fac-dex3 with IPTG promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 829
    Illegal NgoMIV site found at 1162
    Illegal AgeI site found at 1190
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we can use IPTG to induce engineered bacteria expressing fac-dex1.


Source

BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_B0012(ter)

References