Coding
Part:BBa_K2071000:Design
Designed by: Pavle Jeremic Group: iGEM16_UCSC (2016-10-12)
TM1254
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our primary design considerations were to both avoid any RFC10 restriction sites, as well as to codon-optimize the part for translation in Bacillus subtillis
Source
The part originally came from Thermotoga maritima.