Generator

Part:BBa_K2070023:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Constitutive Toehold Trigger generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no TACTAGAG sequence between constitutive promoter sequence and trigger RNA sequence.

Source

Assembly.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, and Peng Yin, (2014, 11) Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell 159, 925–939.