Generator

Part:BBa_K2070014

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-14)


Constitutive antisigma35 generator

This part contains an ECF35 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ECF35 generated can activate the ECF30 promoter (BBa_K2070008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None